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项目简介:
抗肿瘤模型is指用于detectwithevaluate药物抗肿瘤活性of实验模型, 可用于screen抗肿瘤药物.
实验模型of种类繁多, 大致可分for四类:体外试验, 体内试验, 模拟肿瘤of生物模型, 分子生物学模型. 体内肿瘤模型generally常用ofis皮下模型, 腹水 (or尾静脉注射) as well as原位模型.
Experimental Procedure:
测试前需and技术人员联系,需provide待测样品and完善of实验方案 (平台也可according to实验要求出实验方案) , 明确样品浓度and剂量, 给药方式, 动物模型etc.信息.
模型选择: 抗肿瘤模型including体外试验and体内试验两种. 体外试验generally应从敏感及days然耐药of人癌Cell Line中选用至少12种进行试验, 同时考察药物对正常人源cellof影响. 体内试验usuallyadopting动物肿瘤移植模型and人癌移植模型. needaccording to药物特性andresearch目of选择合适of模型.
试验设计: 试验设计应合理, including药物withcell共cultureof时间, 给药方式, 给药剂量, observe时间etc.. for example, 药物withcell共cultureof时间generallyfor48~72hours, 贴壁cell需先贴壁24hours后再给药. in动物模型中, 应in移植肿瘤生长至一定大小后开始给药, such as达到100mm3以上.
control组设置: 试验应设阳性及negative control组. positive controlfor标准抗肿瘤药, negative controlfor溶媒control. inevaluate时还应especially关注受试物withpositive control药试验结果ofrelatively.
safe性research: inevaluate药物抗肿瘤活性of同时, 还need关注药物ofsafe性. for example, in急性毒性试验中进行毒代动力学research, 关注药动学行for差异可能导致of毒性反应of不同.
重复性andreliable性: for了确保结果ofAccuracyandreliable性, 试验至少应重复一times. 此外, for了证明新药可能具have明显of抗肿瘤作用, recommendin尽可能多of模型中evaluate其抑瘤作用.
Data Processingwithanalyze: 对试验数据进行合理ofprocessandanalyze, 提取have用of信息, such as药物对肿瘤cellof增殖抑制率, 凋亡率etc.. 同时, 还need对数据进行统计学analyze, 以评估药物抗肿瘤活性of差异and显著性.
inusing抗肿瘤模型来detectwithevaluate药物抗肿瘤活性时, neednote以下几pcsQuestion:
