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生物膜干涉技术 (Biolayer Interferometry, BLI) 技术is一种免标记技术, 实时provide高通量of生物分子相互作用信息, 具have高Sensitivity, 高detect通量及较低ofSample Requirementsetc.优点. 它不仅can用于早期of研发andscreenas well as晚期of临床试验, 还can用于生产监控and质量监控.
针对市场需求, ACROBiosystemsprovide了相应ofantibody表征, antibodywithFcRs/C1q亲and力detect, 一致性evaluateetc.一系列服务, 以助力于生物医药of发展.
antibodywithFc受体protein亲and力determineaccording toantibodywithantigen亲and力detect, 了解antibodyin体内of动力学特征as well as初步判断药物in体内of代谢etc..
antibodywithFc受体protein亲and力determine
antibodywith补体亲and力determine
任意两种小分子, 其中一pcsneedhave氨基 (所haveofprotein都have) , 带氨基of样品要纯度高, 另一pcs没要求.
providemost少样品量: protein1mg, 小分子100mM/1mL, protein多肽需低温寄送.
Sample Preparation: 样品of质量and纯度对Experimental Results至关important. 确保样品没have污染, 降解或聚集, 并尽量using新鲜制备of样品. 对于protein样品, 可能need进行适当of折叠and纯化, 以确保其具have正确of结构and活性.
传感器选择: BLIusing不同类型of生物传感器来捕获andanalyze分子. according to实验需求选择合适of传感器, such asproteinA传感器, antibody传感器或通用传感器etc.. 确保传感器with待测分子具have适当of亲and力andSpecificity.
实验条件optimize: in进行BLI实验时, needoptimize实验条件以获得most佳结果. 这可能including调整pH值, 离子强度, 温度etc.参数, as well asoptimize样品浓度andincubate时间. through尝试不同of条件组合, 找到most适合你of实验设置.
基线校正: in进行BLI实验时, 基线漂移is一pcs常见ofQuestion. for了获得accurateofExperimental Results, need定期进行基线校正. 这canthroughin实验开始前and结束后record基线信号, 并从实验数据中扣除相应of偏移量来实现.
Data Analysis: BLI实验产生of数据need进行适当ofanalyze以提取have用of信息. 这可能including计算亲and力常数 (KD) , 结合速率 (ka) and解离速率 (kd) etc.参数. using合适of软件或工具进行Data Analysis, 并according toneed进行数据拟合and统计process.
实验Reproducibility: for了确保Experimental Resultsofreliable性, needin不同时间, 不同batchestimesand不同实验人员之间保持实验ofReproducibility. 这要求实验条件, equipment参数and样品process方法etc.都要保持一致, 并且need定期对equipment进行校准and维护.
生物膜干涉技术 (BLI) is一种用于实时监测分子间相互作用of技术, 具have无需标记, 操作简便, detect时间短, 样品耗量少etc.优点. inusingBLI进行分子相互作用analyze时, neednote以下几pcs方面:
