Technical Consultant
One-on-one consultation
Scan QR code to consult
Contact: +86 15884474332
Our Advantages
Professional & Reliable · PhD Expert Team
Credit Payment · Test First, Pay Later
High Cost-effectiveness · Quality & Affordable
Earn Points · Exchange for Rewards
Easy Reimbursement · Official Contract & Invoice
Reports Available · Apply Now
Project Introduction
MALDI-TOF/TOF质谱仪由基质辅助激光解析电离离子源 (MALDI) and飞行时间质量analyze器 (TOF) . MALDI技术is将样品均匀包埋in基质中, 基质吸收激光provideof能量而蒸发, 携带部分样品分子进入气相, 并将一部分能量传递给样品分子使其离子化. TOF原理is离子in电场作用下加速飞过飞行管道, according to达到detect器of飞行时间不同而被detect.
Project Cases
Sample Requirements
关于样品: 关于样品: such as果for胶点样本, 胶点清晰可见; such as果for单一protein样本, 至少need10ugofprotein, 纯度>90% ; 含盐量: 挥发性无机盐<20mM, 不挥发性无机盐<5mM; protein溶液避免SDSetc.变性剂, 表面活性剂, 不含tris缓冲体系.
样品transport: 请using足量of干冰transport, 并尽量选用较快of邮递方式, 以降低transport过程中样品降解of可能性.
FAQ
1,MALDI-TOF/TOF质谱identify适用范围
MALDI-TOF/TOF质谱identifyprotein适用于protein复查程度较低样本, such as2Dprotein胶点, 纯化后protein样品etc.. 全protein样品不isandMALDI-TOF/TOF质谱identify, 可usingLC-MSMS大规模proteinidentify.
