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产品定义
能量代谢维持着生命体most基本of生命活动, 比such as植物逆境and动物疾病usually伴随着严重of代谢紊乱, 且大多归咎于能量代谢紊乱. 能量代谢也影响着免疫cellof功能and命运, with抗病and免疫应答have关. 同时, protein磷酸化of修饰基团来自于ATP, 所以能量代谢异常可进一步导致上游protein磷酸化通路of改变, 严重影响生物体of各项生理, 病理功能. 能量代谢组学方法utilizingbased onLC-MSof靶向代谢组方法对能量代谢相关of30多种代谢物进行绝对定量analyze.
技术路线
技术优势
1. Specificity, 高Accuracy: adoptingMRM技术精确定性, 绝对定量
2. 专属kit: 高通量detect30多种参with能量代谢通路of代谢物
3. 严格质控: 内标+外标, standard人工校对, 标曲R2>0.99
Detection Indicators
参with能量代谢通路of关键30多种代谢物, mainly集中inTCA循环, 糖酵解and氧化磷酸化通路.
Data Analysis
应用领域
糖代谢and能量代谢相关生理and病理research
| Sample Type | 送样量 | 预processandstore |
| 动物tissue | ≥200 mg | PBS wash去除残留血液and污染物, 冲洗干净. 用tissue剪或手术刀将tissue剪切成 1cm3 左右of小块, 样品称重, etc.量分装. 液氮速冻, -80°C store, 干冰transport. |
| Serum | ≥300 μL | 收集好of全血室温静置 30-60 min, or 4°C 放置 2h, 3000 g centrifuge 10 min, 取上清, 液氮速冻, -80°C store, 干冰transport. |
| 血浆 | ≥300 μL | 收集好of全血加入抗凝剂 (推荐adopting肝素抗凝剂, 不recommendadopting柠檬酸or EDTA) , 室温静止 30 minutes, 1300g-2000g centrifuge 10min, 取上清, 液氮速冻, -80°C store, 干冰transport. |
| 尿液 | ≥200 μL | 5000×g 4°C centrifuge 30-60min, 取上清, -80°C store, 干冰transport. |
| 粪便 | ≥300 mg | 新鲜粪便样本收集好后, 立即用液氮速冻process15 min, store至-80℃冰箱 |
| 肠道内容物 | ≥300 mg | 新鲜肠道内容物样本收集好后, 立即用液氮速冻process15 min, store至-80℃冰箱 |
| 乳汁 | ≥200 μL | 收集母乳, -80°C store, 干冰transport. |
| 唾液 | ≥500 μL | 1000g-2000g 4°C centrifuge 10min, (或using 0.22μm 滤膜过滤) , 取上清, -80°C store, 干冰transport. |
| 淋巴液, 脑脊液, 羊水, 胆汁etc.体液 | ≥200 μL | 1000g-2000g 4°C centrifuge 10min, (或using 0.22μm 滤膜过滤) , 取上清, -81°C store, 干冰transport. |
| 穿刺样品 | ≥500 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物种子, 果肉 | ≥200 mg | 收集样品, 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 草本植物, 藻类, 蕨类 | ≥200 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物花粉 | ≥50 mg | 收集样品, 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物体液 | ≥300 μL | 5000×g 4°C centrifuge 30-60min, 取上清, -80°C store, 干冰transport. |
| 软体动物tissue | ≥500 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 低etc.bacteriacell | ≥10^9 | centrifuge收集etc.量菌体 (保证每copies收集of菌体cell数量一致! ! ) , adopting PBS 清洗三times, 每times清洗后 5000g 4°Ccentrifuge 5 minutes, 完全弃去上清, 收集in 1.5ml centrifuge管中. 液氮速冻, -80°C store, 干冰transport. |
| 菌体 (fungus类) | ≥500 mg | 收集菌体 (保证每copies收集of菌体数量or重量一致! ! ) , adopting PBS 清洗三times, 每times清洗后 5000g 4°C centrifuge 5 minutes, 完全弃去上清, 收集in 1.5ml centrifuge管中. 液氮速冻, -80°C store, 干冰transport. |
| 悬浮culturecell | ≥10^7 | centrifuge收集悬浮cell (保证每copies收集ofcell数量一致! ! ) , adopting PBS 清洗两遍, 然后adopting生理盐水 (0.9%氯化钠溶液) 洗一遍, 每times清洗后adopting小于 1000g centrifuge力 4°C 低速centrifuge 3 minutes, 完全弃去上清 (上述整pcs过程需尽量迅速操作) , 收集in 1.5ml centrifuge管中. 液氮速冻后-80°C store, 干冰transport. |
| 贴壁culturecell | ≥10^7 | 收集culture好of贴壁cell (保证每copies收集ofcell数量一致! ! ) 去除干净Culture Medium, adopting预冷of PBS 洗两遍, 然后再times加入预冷ofPBS, 把culture容器中of所havecell都收集下来 (一定要刮取干净! ! ) , 收集in 1.5 mL centrifuge管中, 4°C 低速centrifuge, 完全弃去上清 (上述整pcs过程需尽量迅速操作) . cell沉淀in液氮速冻后-80°Cstore, 干冰transport. |
| Cell Culture上清 | ≥10 mL | 1000 g, 4°Ccentrifuge3 minutes, 取上清, -80°C store, 干冰transport. |
1, 样本制备andprocess: such as何获取高质量, 具have代表性of样本, as well assuch as何process这些样本以most大程度地保留其代谢物信息, is靶向能量代谢组学research中of一pcs关键Question. 不合适of样本制备andprocess方法可能导致代谢物of损失或变化, 从而影响结果ofAccuracy.
2, 代谢物识别and定量: in靶向能量代谢组学research中, 代谢物of识别and定量is关键步骤. 然而, 由于代谢物of种类繁多, 结构复杂, as well as代谢过程中可能存inof同位素标记etc.Question, 使得代谢物ofaccurate识别and定量成for一pcs挑战.
3, Data Analysis: 靶向能量代谢组学researchusually产生大量of数据, such as何对这些数据进行have效analyze并从中提取have用of信息is一pcsimportantQuestion. 数据Analysis Methodof选择, 数据of预process, Statistical Analysisand生物信息学analyzeetc.都isneed考虑of因素.
4, 重复性andreliable性: in靶向能量代谢组学research中, 结果of重复性andreliable性isveryimportantof. such as何确保Experimental Resultsofstable性andreliable性, as well assuch as何process实验中of变异and噪声etc.Question, isresearch中need关注of方面.
5, 生物学意义解释: 靶向能量代谢组学researchof结果need结合生物学背景进行解释. such as何将代谢物变化with特定of生物学过程或疾病机制联系起来, as well assuch as何挖掘潜inof生物标志物或治疗靶点etc., isresearch中need深入探讨ofQuestion.
