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乙酰化(Acetylation)isin乙酰基转移enzymeof作用下, proteinof赖氨酸残基上添加乙酰基of过程, iscell控制gene表达, proteinof活性或生理过程of一种机制. 乙酰化修饰功能mainly集中in对cellstain体结构of影响as well as对核内转录调控因子of激活方面.
应用方向
乙酰化修饰ismost常见of酰化修饰, 参withgene表达调控, cell凋亡, cell代谢, proteinstable性, cell生理状态相关etc.生物学过程, mainly应用领域have: cell转录调控, 代谢通路调控etc..
Experimental Procedure
样品经Trypsinenzyme切, enzyme切产物由乙酰化Specificityantibody进行乙酰化肽段of富集, 富集后由高精度质谱analyze, analyze数据由生物信息学软件进行数据检索.
Sample Type | Sample Requirements |
植物叶片 | 湿重≥0.8g |
植物根茎, 木质部, 韧皮部etc. | 湿重≥10g |
cell样品 | cell量湿重≥8*10⁷ |
tissue样品 | 人, 动物, microorganism湿重≥300mg; |
体液样品 | Serum, 血浆体积≥1ml, , 脑脊液≥1ml, 尿液≥50ml |
Narita T, Weinert BT, Choudhary C. Functions and mechanisms of non-histone protein acetylation [published correction appears in Nat Rev Mol Cell Biol. 2019 Aug;20(8):508]. Nat Rev Mol Cell Biol. 2019;20(3):156-174. doi:10.1038/s41580-018-0081-3
