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through重组DNA (或RNA) 技术获得ofprotein. protein表达系统is指由宿主, 外源gene, 载体and辅助成分组成of体系, through这pcs体系can实现外源genein宿主中表达of目of.
测试狗拥have各类成熟stableof表达平台(酵母, bacteria, 哺乳动物, 昆虫and无cell表达体系), for您provide各类重组proteinof表达服务. be able toprovide: gene合成withoptimize, 载体构建. protein表达条件optimize. protein表达小试with纯化, protein大量生产as well as下游活性detectandprotein结构解析etc.服务, 所制备ofprotein可用于实验室research, 药物screen, 食品卫生, 环境with材料多领域of科学research.
1.目ofgene序列或protein序列及GeneBank登录号, 目ofprotein相关信息.
2．need获得ofprotein及纯度.
3.下单前请with技术人员沟通实验细节.
protein表达量低或无表达:
可能原因: 密码子using不当, gene序列错误, 启动子或终止子不合适, 表达条件不合适etc..
解决方案: optimize密码子using, 检查gene序列, 选择合适of启动子and终止子, 调整表达条件.
protein溶解性差:
可能原因: proteinof折叠或stable性Question, Culture Medium成分不合适, 表达温度过高或过低etc..
解决方案: optimizeproteinof折叠andstable性, 调整Culture Medium成分, 选择合适of表达温度.
protein纯化困难:
可能原因: proteinwith杂质结合紧密, 纯化方法不合适, 纯化条件不optimizeetc..
解决方案: optimize纯化方法, 调整纯化条件, using标签protein进行纯化etc..
protein活性低或丧失:
可能原因: 纯化过程中protein变性, store条件不合适, protein修饰缺失etc..
解决方案: optimize纯化过程, 选择合适ofstore条件, 确保proteinof正确修饰.
protein聚集或沉淀:
可能原因: protein浓度过高, pH值不合适, 离子强度不合适etc..
解决方案: 降低protein浓度, 调整pH值and离子强度, using添加剂such as去垢剂etc..
内毒素污染:
可能原因: using大肠杆菌etc.含have内毒素of宿主cell进行表达.
解决方案: using低内毒素产生of宿主cell, 进行内毒素去除process.
