One-on-one consultation
Scan QR code to consult
Contact: +86 15884474332
Professional & Reliable · PhD Expert Team
Credit Payment · Test First, Pay Later
High Cost-effectiveness · Quality & Affordable
Earn Points · Exchange for Rewards
Easy Reimbursement · Official Contract & Invoice
Reports Available · Apply Now
产品定义
protein磷酸化修饰 (Phosphorylation) is生物体内mostimportantof共价修饰方式之一, is目前分布most多, 也ismost广泛researchof修饰, 其发生过程isin激enzyme (kinase) 催化下, 将ATPof磷酸根基团转移到proteinof氨基酸 (Ser,Tyr,Thr) 侧链上. proteinof磷酸化and去磷酸化这一可逆过程调节着includingcell增殖, 发育, 分化, 信号转导, cell凋亡, 神经活动, 肌肉收缩及肿瘤发生etc.过程in内of所have生命活动. 发生磷酸化修饰of氨基酸上共价连接磷酸基团, 其分子质量会增加发生79.97Da. utilizing质谱可determine分子量is否发生79.97Daof质量偏移, 从而detect磷酸化修饰肽段及位点.
4D-label free磷酸化protein组学isbased ontimsTOF Pro质谱仪, through数据依赖性采集-同步累积连续碎裂 (ddaPASEF) 扫描模式进行差异磷酸化protein组学analyze.4D-label free修饰组, utilizing了离子淌度分离, 能according to分子of形状, 截面积属性, 可have效区分修饰同分异构多肽, 极大提高修饰ofidentify深度, 以更少上样量, 更快扫描速度, 实现protein组学in覆盖深度, Sensitivity, 通量方面of全面性提升.
技术路线
技术优势
1. 4D技术加持, 提高定量ofAccuracy, identify深度, more适合微量样本;
2. 克服标记定量protein组学技术in样本数量上of限制, 灵活convenient;
3. based on质谱平台 (Bruker timsTOF pro) , detect速度提升近一倍;
4. 经验丰富- -超17年protein平台服务经验, 拥have丰富of高水平项目文章;
5. 数据保证- -adopting进口金标antibody, 数据结果stablereliable;
6. 系统解决方案- -provide修饰组学至validate, 上下游联合analyze, 实现快捷”一站式”服务.
推荐应用领域
1. 神经及退行性疾病;
2. 肿瘤发病机制research;
3. 药理药靶research.
Data Analysis
推荐组合产品
1. protein组+磷酸化组: 全面剖析机体调控网络.
2. 磷酸化组+代谢组: 打通机体表型-机制research通路.
参考文献
1.Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis. 2020. Cell Metabolism. (IF 27.287) [client文献]
adopting磷酸化修饰组学,DIAprotein组学etc.发现低剂量索拉菲尼可改善非酒精性脂肪肝炎并揭示其作用机制
2.Synergistically Bifunctional Paramagnetic Separation Enables Efficient Isolation of Urine Extracellular Vesicles and Downstream Phosphoproteomic Analysis. 2021. ACS Appl Mater Interfaces. (IF 9.229)
4D-label freeprotein组+4D磷酸化protein组学应用于前列腺癌患者标志物research
3.The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis.2020. Autophagy. (IF 16.016) [client文献]
磷酸化Label freeprotein组助力research动脉粥样硬化of潜in治疗靶标
4.Protein phosphatase 2A has an essential role in promoting thymocyte survival during selection. 2019. PNAS. (IF 11.205) [client文献]
磷酸化Label freeprotein组researchPP2A介导protein去磷酸化of关键调控作用
