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产品定义
中长链脂肪酸is指碳原子数量大于6以上of脂肪酸, 它们不但be able tofor生物体生命活动provide能量, 同时也isimportantof信号分子. utilizing靶向(GC-SIM)ofanalyze技术进行detect, 对生物样本中of40种中长链脂肪酸进行绝对定量analyze.
技术路线
技术优势
1. 高Specificity, 高Accuracy: adoptingGC-SIM精确定性, 内标法绝对定量
2. 专属kit: 高通量detect40种中长链脂肪酸
3. 严格质控: 多重质控, standard人工校对, 标曲R2>0.99
Detection Indicators
Data Analysis
应用领域
营养发育, 肿瘤, 代谢综合征, 植物生理etc.research
| Sample Type | 送样量 | 预processandstore |
| 动物tissue | ≥200 mg | PBS wash去除残留血液and污染物, 冲洗干净. 用tissue剪或手术刀将tissue剪切成 1cm3 左右of小块, 样品称重, etc.量分装. 液氮速冻, -80°C store, 干冰transport. |
| Serum | ≥300 μL | 收集好of全血室温静置 30-60 min, or 4°C 放置 2h, 3000 g centrifuge 10 min, 取上清, 液氮速冻, -80°C store, 干冰transport. |
| 血浆 | ≥300 μL | 收集好of全血加入抗凝剂 (推荐adopting肝素抗凝剂, 不recommendadopting柠檬酸or EDTA) , 室温静止 30 minutes, 1300g-2000g centrifuge 10min, 取上清, 液氮速冻, -80°C store, 干冰transport. |
| 尿液 | ≥200 μL | 5000×g 4°C centrifuge 30-60min, 取上清, -80°C store, 干冰transport. |
| 粪便 | ≥300 mg | 新鲜粪便样本收集好后, 立即用液氮速冻process15 min, store至-80℃冰箱 |
| 肠道内容物 | ≥300 mg | 新鲜肠道内容物样本收集好后, 立即用液氮速冻process15 min, store至-80℃冰箱 |
| 乳汁 | ≥200 μL | 收集母乳, -80°C store, 干冰transport. |
| 唾液 | ≥500 μL | 1000g-2000g 4°C centrifuge 10min, (或using 0.22μm 滤膜过滤) , 取上清, -80°C store, 干冰transport. |
| 淋巴液, 脑脊液, 羊水, 胆汁etc.体液 | ≥200 μL | 1000g-2000g 4°C centrifuge 10min, (或using 0.22μm 滤膜过滤) , 取上清, -81°C store, 干冰transport. |
| 穿刺样品 | ≥500 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物种子, 果肉 | ≥200 mg | 收集样品, 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 草本植物, 藻类, 蕨类 | ≥200 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物花粉 | ≥50 mg | 收集样品, 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 植物体液 | ≥300 μL | 5000×g 4°C centrifuge 30-60min, 取上清, -80°C store, 干冰transport. |
| 软体动物tissue | ≥500 mg | 收集样品, adopting PBS wash去除样品表面泥土或污染物 (务必清洗干净! ! ) , 样品称重, etc.量分装, 液氮速冻, -80°C store, 干冰transport. |
| 低etc.bacteriacell | ≥10^9 | centrifuge收集etc.量菌体 (保证每copies收集of菌体cell数量一致! ! ) , adopting PBS 清洗三times, 每times清洗后 5000g 4°Ccentrifuge 5 minutes, 完全弃去上清, 收集in 1.5ml centrifuge管中. 液氮速冻, -80°C store, 干冰transport. |
| 菌体 (fungus类) | ≥500 mg | 收集菌体 (保证每copies收集of菌体数量or重量一致! ! ) , adopting PBS 清洗三times, 每times清洗后 5000g 4°C centrifuge 5 minutes, 完全弃去上清, 收集in 1.5ml centrifuge管中. 液氮速冻, -80°C store, 干冰transport. |
| 悬浮culturecell | ≥10^7 | centrifuge收集悬浮cell (保证每copies收集ofcell数量一致! ! ) , adopting PBS 清洗两遍, 然后adopting生理盐水 (0.9%氯化钠溶液) 洗一遍, 每times清洗后adopting小于 1000g centrifuge力 4°C 低速centrifuge 3 minutes, 完全弃去上清 (上述整pcs过程需尽量迅速操作) , 收集in 1.5ml centrifuge管中. 液氮速冻后-80°C store, 干冰transport. |
| 贴壁culturecell | ≥10^7 | 收集culture好of贴壁cell (保证每copies收集ofcell数量一致! ! ) 去除干净Culture Medium, adopting预冷of PBS 洗两遍, 然后再times加入预冷ofPBS, 把culture容器中of所havecell都收集下来 (一定要刮取干净! ! ) , 收集in 1.5 mL centrifuge管中, 4°C 低速centrifuge, 完全弃去上清 (上述整pcs过程需尽量迅速操作) . cell沉淀in液氮速冻后-80°Cstore, 干冰transport. |
| Cell Culture上清 | ≥10 mL | 1000 g, 4°Ccentrifuge3 minutes, 取上清, -80°C store, 干冰transport. |
样本量: 确保样本量大于0.2gor0.2mL, 这is进行GC-MSanalyzeof基本要求, 以确保足够of物质供instrumentdetect.
样本process: 由于长链脂肪酸不易挥发, 因此可能need进行衍生化process, such as酯化或硅烷化, 以增加其挥发性, 便于GC-MSdetect.
instrument设置: according to长链脂肪酸of特性, need选择合适of色谱柱anddetect器, as well as适当ofanalyze条件, such as温度, 载气流速etc., 以确保长链脂肪酸ofhave效分离anddetect.
Quality Control: for了保证analyze结果ofAccuracy, 应进行严格ofQuality Control, includingusingstandard进行定量analyze, 定期维护and校准instrument, as well as重复determine样品etc..
数据解析: GC-MSanalyze会产生大量of数据, need仔细解析, including识别色谱峰, 计算峰面积, 确定长链脂肪酸of种类and浓度etc.. 这needhave经验ofanalyze师进行.
